This master thesis was written during an internship from april 1 till october 30, 2007 at the karolinska institute 10 3 materials and methods 11 31 materials 11 311 cell lines and plasmids 11 312 antibodies 12 313 a2ar agonists and antagonists 13 362 sds-page and western blotting 21. Temed tetramethyl-ethylenediamin tm protein transmembrane envelope protein t-rna transfer-ribonucleic acid wb western blot vlp virus like particles bachelor thesis alexander šach robert koch institute 2 material and methods 21 material 211 antibiotics name vendor catalog number. I hereby confirm that my phd thesis entitled “in vivo characterization of genetic factors involved in xmrk driven melanoma formation in medaka (oryzias latipes): a closer look at braf, stat5 and c-myc” is the result of my own work i did not receive any help or support from commercial consultants all sources and/or materials. 28 2 materials and methods 21 materials 211 commercial suppliers unless otherwise stated, all chemicals were purchased from merck, sigma-aldrich difco western blot the pvdf membrane of the western blot was stained with coomassie until blue protein bands were visible and the membrane was air dried. Early efforts to develop the methods for evaluating the effects of the q141k mutant material subsequently, phase separation was achieved using 40 ul of chloroform per tube and the upper aqueous phase was transferred to a fresh 15 ml centrifuge figure 3: western blot analysis using different detection methods.
Cancer alaa alshareeda july 2014 thesis submitted to the university of nottingham for the degree of doctor of philosophy method: the study cohort comprises three bc groups: a) large series of unselected primary sporadic operable invasive tumours 2144 specificity of antibodies by western blot ( wb). Lars funke, phd thesis material and methods - 69 - material and methods dna constructs neurons were then lysed with sds-page sample buffer lysates were analyzed with sds-page and western blot analysis blots were probed with psd-95, psd-93, sap97, and sap102 as described above as well. Dissertation submitted to the combined faculties for the natural sciences and for mathematics of the ruperto-carola university of heidelberg, germany for the degree of doctor of natural sciences presented by master of science hannah katarina schneider born in tübingen, germany oral-examination. Freia spillmann phd thesis material and methods 100 protein western blot cells were washed twice with cold pbs, counted, resuspended and lysed in an appropriate volume of lysis buffer (tab 1) for 20 min on ice as a ratio cell/lysis buffer: approximately 2-3 x 107 cell per 1 ml lysis buffer was taken samples were.
Dissertation submitted to the combined faculties for the natural sciences and for mathematics of the ruperto-carola university of heidelberg, germany for the degree of doctor of natural sciences presented by diplom-biologin doris quinkert materials and methods 2219 northern blot analysis. Abscisic acid and desiccation stress dissertation zur erlangung des doktorgrades (dr rer nat) der mathematisch-naturwissenschaftlichen fakultät der rheinischen materials and methods 23 21 plant material western blot analysis on c plantagineum total protein from callus 109 347 overview of the.
Chapter 2 materials and methods bio-rad bradford protein quantification acrylamide gels clontech luciferase reporter assay human mtn blot i human mtn blot ii fuji solutions used in this thesis are listed below, alphabetically within each section 243 electrophoresis solutions and western blotting solution. Materials and methods 2 materials and methods 21 materials 21 1 bacterial strains and cell lines bacteria dh5α ecoli k12, f- enda1 transferred onto nitrocellulose membranes for western blot analysis membrane (schleicher & schuell) by semi-dry blotting (harlow and lane, 1988) in 1 x. Chapter 2 materials and methods 61 2 materials and methods 21 materials 211 reagents all chemicals were obtained from sigma (poole, dorset, uk) or bdh selective media and multiple colonies were selected and screened by colony blots for western blot analysis before being stored in microbanks at - 80oc. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly our laboratory investigated, via western blotting, blm protein levels during adenovirus infection preliminary results from these investigations 14 chapter two: materials and methods.
In this project, immunoblotting techniques (western blots) and mature fractionation methods were utilized hupl specific antibodies and vegetative cells, which provide the basic method for separating these two kinds of cells the modalities of them can be materials and methods 21 strains, culture conditions and. Chapter 2 materials and methods 22 the experiment in this thesis is set out to learn and understand the biology or chemistry basis 26 western blotting after electrophoresis, the proteins in the gel were transferred to a hybond-ecl nitrocellulose membrane (by ge healthcare) using a blotting system as. Proteoglycan characterization in cog7-deficient cells tomasz konopka master thesis department of biosciences the faculty of mathematics and natural vesicular stomatitis virus glycoprotein vt terminal volume vtm vesicular transport model wb western blotting xyl xylose 35 s-so4 2- radioactive 35.